The second journey into the world of Desmond Miles, ancestry of the legendary assassin Altair.

Desmond start by seeing the glyph left behind by Subject 16 in his room, inside Abstergo lab. After knowing what Abstergo Lab, part of Templar community, want to do with Piece of Eden, he start to worry what will happen if he truly find out about location of Piece of Eden. Luckily he have a friend Lucy, his doctor and also memberof Assassin in present day, that help him get out of Abstergo Lab and join her Assassin community. Before they left, she steal data from the lab about Desmond other ancestor, Ezio Auditore, to find out about Piece of Eden location before The Templar find out first.